The Small GTPase Ran

Inhaltsverzeichnis

• 1. The Role of Ran in Nuclear Import.
• 1. Introduction.
• 2. The Ran GTPase cycle and how it relates to nuclear protein import.
• 3. Concluding remarks.
• 4. References.
• 2. Ran GTPase Regulation of the CRMl-Dependent Export Pathway.
• 2. Nuclear export signals.
• 3. Identification of CRMl/exportin 1, the receptor for leucine-rich NES.
• 4. Assembly of nuclear export complexes.
• 5. Docking and translocation of the export complex through the NPC.
• 6. Release of the export complex from the NPC.
• 7. Concluding remarks.
• 8. References.
• 3. Role of Ran GTPase in RNA Processing and Export of RNA from the Nucleus to the Cytosol: Insights from Budding Yeast.
• 2. Ran pathway and tRNA biogenesis in S. cerevisiae.
• 3. Ran and its role in ribosome biogenesis and nuclear export of ribosomes in S. cerevisiae.
• 4. Ran and its role in nuclear export of mRNAs in S. cerevisiae.
• 5. Concluding remarks.
• 6. Acknowledgements.
• 7. References.
• 4. Direct and Indirect Roles of Ran·GTP in Nuclear Export of RNAs in Higher Eukaryotes.
• 2. Export of tRNA.
• 3. Export of pre-snRNAs.
• 4. Export of mRNAs.
• 5. Export of ribosomal RNAs.
• 6. Concluding remarks.
• 7. Acknowledgements.
• 5. The Role of Ran in Regulating Microtubule Spindle Assembly.
• 2. Studies that implicated Ran in the regulation of the microtubule cytoskeleton.
• 3. Ran-GTP can stimulate microtubule spindle assembly in vitro.
• 4. Generation of Ran-GTP by chromatin-bound RCC1 is required for spindle assembly.
• 5. The molecular mechanism by which Ran regulates spindle assembly.
• 6. Which spindle-forming processes does Ran regulate?.
• 7. A model for how Ran-GTP regulates microtubule spindle assembly.
• 8. Does Ran play a role in spindle assembly in vivo?.
• 9. Could Ran-GTP have functions other than regulating spindle formation in mitosis?.
• 10. Concluding remarks.
• 11. Acknowledgements.
• 12. References.
• 6. Multiple Roles of the Ran GTPase During the Cell Cycle.
• 2. Expression of Ran pathway components in development and the cell cycle.
• 3. Post-translational regulation of the Ran pathway.
• 4. Ran’s role in regulation of the onset and completion of mitosis.
• 5. Ran and mitotic spindle assembly.
• 6. Ran and post-mitotic nuclear assembly.
• 7. Concluding remarks, future directions.
• 8. Acknowledgements.
• 9. References.
• 7. The Multiple Roles of Ran in the Fission Yeast Schizosac-charomyces pombe.
• 2. Introduction to the fission yeast S. pombe.
• 3. Discovery of S. pombe Ran components.
• 4. Localization of S. pombe Ran components.
• 5. Phenotypic consequences of perturbing the Ran system.
• 6. Evidence that the Ran system has multiple functions in fission yeast.
• 7. Comparison of the phenotypic consequences of perturbing the Ran system in S. pombe and other experimental systems.
• 8. Concluding remarks: Unanswered questions and future directions regarding the role of Ran in S. pombe.
• 9. Acknowledgements.
• 10. References.
• 8. Mitotic Control by Ran and RanBPl in Mammalian Cells.
• 2. Mammalian RanBPl protein: structure and functions.
• 3. Localization of components of the Ran network during the cell cycle.
• 4. Spindle assembly in mammalian cells.
• 5. Mitotic division.
• 6. Nuclear reconstitution at mitotic exit.
• 9. Ran Regulation by Ran GEF and Ran GAP.
• 2. Ran GEF stimulates guanine nucleotide exchange, and the resulting active Ran-GTP terminates import into the nucleus.
• 3. In the cytoplasm, RanGAP induces Ran GTPaseactivity….
• 4.…with some help from RanBP2 and RanBPl.
• 5. Mogl induces release of GTP from Ran.
• 10. Structural Views of the Ran GTPase Cycle.
• 2. Ran·GTP and Ran-GDP, active and inactive conformations.
• 3. NTF2, a binding partner of Ran·GDP.
• 4. Activation: RCC1 and its complex with Ran.
• 5. Deactivation: Ran·GAP.
• 6. Effectors of Ran.
• 8. Acknowledgments.