Thermodynamics of Glycolysis von Thorsten Greinert | ISBN 9783843946322

Thermodynamics of Glycolysis

von Thorsten Greinert
Buchcover Thermodynamics of Glycolysis | Thorsten Greinert | EAN 9783843946322 | ISBN 3-8439-4632-9 | ISBN 978-3-8439-4632-2

Thermodynamics of Glycolysis

von Thorsten Greinert
Thermodynamic feasibility analyses help evaluating the feasibility of metabolic pathways, which is an important information used to develop new biotechnological processes and to understand metabolic processes in cells. However, literature standard data are uncertain for most biochemical reactions yielding wrong statements concerning their feasibility.
In this work, thermodynamics of glycolytic reactions triosephosphate isomerase, glyceraldehyde 3-phosphate dehydrogenase and enolase were investigated. The experimental investigation of the influence of substrate concentrations as well as crowders and salts on the reaction equilibria showed that they depend strongly on these reaction conditions. These influences were accounted for by means of activity coefficients, determined with the equation of state ePC-SAFT in order to gain new standard Gibbs energy of reaction values for the three experimentally investigated reactions as well as for the glycolytic reactions phosphofructokinase, aldolase and phosphoglycerate mutase.
As a result, activity-based standard values for all glycolytic reactions are available and a feasibility analysis was performed. This showed that salts as well as the concentrations of all metabolites within the pathway have a significant influence on the feasibility of the single glycolytic reactions. It was possible to correctly determine the feasibility of the glycolytic pathway.