Regulation of the RAS Signalling Network

Inhaltsverzeichnis

• 1. Genetics of RAS Signaling in Drosophila.
• I. Introduction.
• II. Background to the Experimental Systems.
• III. Protein Tyrosine Kinase Receptors.
• IV. Linking RAS with Tyrosine Kinase Receptors.
• V. A Kinase Cascade Downstream of RAS.
• VI. Nuclear Events.
• VII. Concluding Remarks.
• 2. RAS-Mediated Signal Transduction in C. elegans.
• II. Genetic Approaches Used to Study Vulval Signal Transduction.
• III. Genetic and Molecular Analysis of the let-60 RAS Gene.
• IV. Genes Acting Upstream of RAS.
• V. Genes Acting Downstream of RAS.
• VI. Multiple Functions of the let-60 RAS-mediated Signal Transduction Pathway During C elegans Development.
• 3. Mammals I: Regulation of RAS Activation.
• II. Growth Factor/Cytokine Signaling.
• III. Growth Factor Cascades: Autocrine Stimulation.
• 4. Prenylation of RAS and Inhibitors of Prenyltransferases.
• II. C-Terminal Modification of RAS Proteins.
• III. Farnesyltransferase and Geranylgeranyltransferases.
• IV. Mutational Analyses of Prenyltransferases.
• V. Inhibitors of Prenylation.
• VI. Biological Effects of Prenyltransferase Inhibitors.
• 5. Mammals II: Downstream of RAS and Actin-Cytoskeleton.
• I. RAS GAPs.
• II. Oncogenic Mutations of RAS.
• III. Effectors of RAS.
• IV. Actin-Cytoskeleton.
• V. G Proteins in the Rho Family (Rho, Rac and CDC42).
• VI. RAS-Activated or -Repressed Genes.
• 6. From RAS to MAPK: Cell-Free Assay System for RAS- and Rap 1-Dependent B-Raf Activation.
• II. Cell-free Assay System for the RAS-dependent Activation of the MAP Kinase Cascade in Xenopus Oocyte Cytosol.
• III. REKS.
• IV. Raf Activation.
• V. Conclusion.