Advanced Research on Animal Cell Technology

Inhaltsverzeichnis

• I. Cell-Cell Communication and Cell Extracellular Matrix Interactions.
• Extracellular Matrix Cell Differentiation.
• Structure and Function of the Heparan Sulfate Proteoglycans.
• Role Played by Gap Junctions in Cell-Cell Communication.
• Fibronectin-Mediated Cellular (Inter)actions.
• II. “Normal” Versus “Abnormal” Cells.
• Use of Cell Lines in Biotechnology.
• Why Not Use Primary Cells.
• III. Large Scale Cultivation of Animal Cells.
• Technology of Mass Cultivation: An Overview.
• Cell Culture in Hollow Fibre Bioreactors.
• Perfusion Culture.
• Good Manufacturing Practice in the Production of Biologicals by Cell Cultures.
• Biosensors.
• IV. Analysis of the Cell’s Behaviour.
• Effects of Retinoids on Growth Differentiation.
• Phosphoinositide Metabolism and Control of Cell Proliferation.
• Somatic Cell Genetic Analysis of Growth Control.
• Promoter Organization of Eukaryotic Protein-Coding Genes.
• V. Modification of the Cell Genome.
• Tailoring of an Anti-Human Placental Alkaline Phosphatase Immunoglobulin Using Genetic Engineering.
• Vaccinia Virus as an Expression Vector.
• Expression and Integration of Exogenous DNA Seguences Transfected into Mammalian Cells.
• Cooperation of Oncogenes in Malignant Transformation.
• VI. Applications of Animal Cell Cultures.
• Molecular Dissection of the Cell Cycle.
• Use of Microbeads for Cell Transplantation.
• Influence of Epigenetic Factors on Epidermal Differentiation and Morphogenesis.
• The Characterization of Fibroblast Growth Factor and Its Biological Effect In Vitro and In Vivo.
• Culture of Hybridomas — A Survey.
• Applications of Chromatography in Downstream Processing.
• General discussion.