Molecular Biophysics of the Extracellular Matrix

Inhaltsverzeichnis

• 1 Structure and Associations of Proteoglycans in Cartilage.
• Structure.
• Preparation.
• Link-Free Aggregates.
• Link-Stable Aggregates.
• The Secretion and Assembly of Proteoglycan Aggregates.
• Diversity of Proteoglycan Structure.
• 2 The Dilute Solution Properties of Glycosaminoglycans and Proteoglycans.
• The Relevance of the Term “Dilute Solution”.
• Hyaluronate.
• The Sulfated Glycosaminoglycans.
• Proteoglycans.
• Conclusions.
• 3 X-Ray Diffraction Analyses of Glycosaminoglycans.
• The Relevance of Fiber Diffraction Studies.
• Accuracy and Precision in Fiber Diffraction Analyses.
• Polymorphism of Glycosaminoglycan Helices.
• Detailed Structural Studies of Hyaluronate Allomorphs.
• Detailed Structural Studies of Chondroitin Sulfate Allomorphs.
• Detailed Structural Studies of Dermatan Sulfate Allomorphs.
• The Roles of Cations in Conformation and Packing.
• Proteoglycans and Heterotypic Interactions.
• 4 Conformation of Individual Residues and Chain Segments of Glycosaminoglycans in Solution by Spectroscopic Methods.
• Conformation of Individual Residues.
• Conformation of Chain Segments.
• 5 Self-Association of Copolymeric Glycosaminoglycans (Proteoglycans).
• Dermatan Sulfate.
• Heparan Sulfate.
• Proteodermatan Sulfate.
• 6 Transport of Molecules in Connective Tissue Polysaccharide Solutions.
• The State of Polysaccharides in Connective Tissue.
• Modes of Transport.
• Rotational Diffusion.
• Transport of Electrolytes.
• Formation and Transport of Matrix Structures.
• 7 Glycosaminoglycan Chains in the Biological State.
• Introduction—Molecular Species.
• Molecular Shapes.
• Molecular Assemblies.
• References.