Methods of Protein Microcharacterization

Inhaltsverzeichnis

• Microisolation Techniques.
• Microisolation of Biologically Active Polypeptides by Reverse-Phase Liquid Chromatography.
• Reverse-Phase HPLC Isolation and Microsequence Analysis.
• Purification of Microgram Quantities of Proteins by Polyacrylamide Gel Electrophoresis.
• Microamino Acid Analysis.
• Amino Acid Analysis With Postcolumn Fluorescent Derivatization.
• Amino Acid Analysis by o-Phthaldialdehyde Precolumn Derivatization and Reverse-Phase HPLC.
• Amino Terminal Analysis.
• Manual Edman Sequencing System.
• Comparison of the Spinning Cup and Gas-Phase Instruments.
• Gas-Phase Protein/Peptide Sequencer.
• Design of a Multipurpose Sequencer.
• Solid-Phase Methods in Protein Microsequence Analysis.
• Analysis of Phenylthiohydantoin Amino Acids by HPLC.
• Purification and Preparation of Chemicals for Microsequencing With the Edman Degradation.
• Carboxyl-Terminal Analysis.
• Microsequence Analysis by Enzymatic Methods.
• COOH-Terminal Analysis of Polypeptides by Chemical and Chromatographic Methods.
• Mass Spectrometric Analysis of Polypeptides.
• Polypeptide Sequence Analysis Using Gas Chromatography-Mass Spectrometry.
• Fast Atom Bombardment and Secondary Ion Mass Spectrometry of Peptides and Proteins.