Dieser Titel wurde ersetzt durch:
- Handbook of ELISPOT (978-1-61779-324-0) - Einband - fest (Hardcover)
×
![]( "Buchcover ISBN 9781588294692")
From the reviews:
„The book is divided into four sections, with a total of 20 chapters featuring step-by-step protocols that will enable both novice and experienced researchers to perform ELISPOT. Each chapter begins with a general Introduction, followed by a detailed Materials section and an easy-to-follow Methods section … . All the chapters are equally interesting and well presented … . a must for a cytochemist. This book will serve as a convenient reference manual for beginners and for experienced ELISPOT users.“ (Carlo Alberto Redi, European Journal of Histochemistry, December, 2005)
In this first book dedicated entirely to the ELISPOT, a critical enzyme-linked immunospot assay used widely in biomedical research, recognized experts with first-hand experience detail how to design, perform, and analyze these assays. The readily reproducible techniques they provide cover a wide variety of topics, including the use of membrane-backed plates, the standardization and validation procedures, the removal of cells from ELISPOT plates, cell separation techniques, and the quantification of ELISPOT data. There are also numerous ELISPOT applications involving animal models, human cells, measles, multiple sclerosis, immune responses, multicytokine detection systems, and immunocytochemistry. Highlights include dual-color and multiplex ELISPOT assays, use of the ELISPOT assay on feline lymphocytes, standardization of the ELISPOT procedure, and combining the ELISPOT assay with immunohistochemistry.
