Microinjection | ISBN 9783764360191

Microinjection

herausgegeben von Juan Carlos Lacal, Rosario Perona und James Feramisco
Mitwirkende
Herausgegeben vonJuan Carlos Lacal
Herausgegeben vonRosario Perona
Herausgegeben vonJames Feramisco
Buchcover Microinjection  | EAN 9783764360191 | ISBN 3-7643-6019-4 | ISBN 978-3-7643-6019-1

Microinjection

herausgegeben von Juan Carlos Lacal, Rosario Perona und James Feramisco
Mitwirkende
Herausgegeben vonJuan Carlos Lacal
Herausgegeben vonRosario Perona
Herausgegeben vonJames Feramisco

Inhaltsverzeichnis

  • 1. Needle Microinjection: A Brief History.
  • 2. Microinjection of Macromolecules into Mammalian Cells in Culture.
  • 3. The Use of Plasmid Microinjection to Study Specific Cell Cycle Phase Transitions.
  • 4. Combining Microinjection with Microanalytical Methods to Investigate Regulation of Metabolic Pathways.
  • 5. Import of Stably Folded Proteins into Peroxisomes.
  • 6. Study of the Function of Microtubule Proteins.
  • 7. Cytoskeleton Regulation by Rho Proteins.
  • 8. Cytoskeletal Regulation by Rho-p21 Associated Kinases: Analysis of ROK and PAK Function by Plasmid Microinjection.
  • 9. Use of Microinjection to Study Apoptosis and its Prevention.
  • 10. Role of the InsP3 Receptor in Intracellular Ca2+ Release and Ca2+ Entry.
  • 11. Microinjection of Xenopus laevis Oocytes: A Model System.
  • 12. Xenopus laevis Oocytes as a Model for Studying the Activation of Intracellular Kinases.
  • 13. Signal Transduction in Xenopus laevis Oocytes by Ras Oncoproteins and Lipid Metabolites.
  • 14. The Multiple Roles of Mos During Meiosis.
  • 15. Use of the Xenopus Oocyte System to Study RNA Transport.
  • 16. Functional Expression of G Protein-Coupled Receptors in Xenopus laevis Oocytes.
  • 17. Expression of Glucose Transporters in Xenopus laevis Oocytes: Applications for the Study of Membrane Transporter Function and Regulation.
  • 18. DNA Injection into Xenopus laevis Embryos as a Tool to Study Spatial Gene Activity.
  • 19. Transformation of Nematodes by Microinjection.
  • Guide to Solutions.
  • Guide to Protocols.
  • Troubleshooting Guide.