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![]( "Buchcover ISBN 9780470748596")
„Chapters cover key biotransformations from the literature, detailing the procedures used, the sources of the startingmaterials and reagents, references, and tips and advice, whereappropriate.“ (JACS, 2010)
"It starts with a review of the current use of biocatalysis inthe pharmaceutical industry. Although this is focused on a largepharmaceutical company (GlaxoSmithKline), it covers a wide varietyof aspects of the industry and offers insightful comparisons ofbiocatalysis with 'traditional' organic synthesis.„(Chemistry World, July 2010)
“I can recommend to all those who are interesting in carryingout biotransformations in the laboratory, and the book should helppersuade those who have never used a biotransformation in anorganic synthesis, to try one for the first time." (OrganicProcess Research & Development Journal, 2010)
Practical Methods for Biocatalysis and Biotransformations
herausgegeben von John Whittall und Peter W. SuttonBiocatalysts are increasingly used by chemists engaged in finechemical synthesis within both industry and academia. Today, thereexists a huge choice of high-tech enzymes and whole cellbiocatalysts, which add enormously to the repertoire of syntheticpossibilities.
Practical Methods for Biocatalysis and Biotransformationsis a „how-to“ guide focussing on commercially availableenzymes and strains of microorganisms that are readily obtainedfrom culture collections. The source of starting materials andreagents, hints, tips and safety advice (where appropriate) aregiven to ensure, as far as possible, that the procedures arereproducible. Comparisons to alternative methodology are given andrelevant references to the primary literature are cited. Contentsinclude:
* Introduction to the application of biocatalysts in the pharmaindustry
* introduction to molecular biological techniques for biocatalystdevelopment
* Lipase and protease methods including enzyme supports anddynamic kinetic resolutions
* Nitrile hydrolases and hydratases
* Alcohol dehydrogenases and co-factor recycling
* Epoxide hydrolases
* Amino acid synthesis
* Whole cell processes
* Other biocatalysts, including monoamineoxidases, aminotransterases, and lyases
Practical Methods for Biocatalysis and Biotransformationsis an essential text offering validated biocatalyst methods forresearchers and postgraduate students in industry and academia.
Practical Methods for Biocatalysis and Biotransformationsis a „how-to“ guide focussing on commercially availableenzymes and strains of microorganisms that are readily obtainedfrom culture collections. The source of starting materials andreagents, hints, tips and safety advice (where appropriate) aregiven to ensure, as far as possible, that the procedures arereproducible. Comparisons to alternative methodology are given andrelevant references to the primary literature are cited. Contentsinclude:
* Introduction to the application of biocatalysts in the pharmaindustry
* introduction to molecular biological techniques for biocatalystdevelopment
* Lipase and protease methods including enzyme supports anddynamic kinetic resolutions
* Nitrile hydrolases and hydratases
* Alcohol dehydrogenases and co-factor recycling
* Epoxide hydrolases
* Amino acid synthesis
* Whole cell processes
* Other biocatalysts, including monoamineoxidases, aminotransterases, and lyases
Practical Methods for Biocatalysis and Biotransformationsis an essential text offering validated biocatalyst methods forresearchers and postgraduate students in industry and academia.
