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Examining the physical basis of the structure ofmacromolecules--proteins, nucleic acids, and theircomplexes--using calorimetric techniques
Many scientists working in biology are unfamiliar with thebasics of thermodynamics and its role in determining molecularstructures. Yet measuring the heat of structural change a moleculeundergoes under various conditions yields information on theenergies involved and, thus, on the physical bases of theconsidered structures. Microcalorimetry of Macromoleculesoffers protein scientists unique access to this importantinformation.
Divided into thirteen chapters, the book introduces readers tothe basics of thermodynamics as it applies to calorimetry, theevolution of the calorimetric technique, as well as howcalorimetric techniques are used in the thermodynamic studies ofmacromolecules, detailing instruments for measuring the heateffects of various processes. Also provided is general informationon the structure of biological macromolecules, proteins, andnucleic acids, focusing on the key thermodynamic problems relatingto their structure. The book covers:
* The use of supersensitive calorimetric instruments, includingmicro and nano-calorimeters for measuring the heat of isothermalreactions (Isothermal Titration Nano-Calorimeter), the heatcapacities over a broad temperature range (ScanningNano-Calorimeter), and pressure effects (Pressure PerturbationNano-Calorimeter)
* Two of the simplest but key structural elements: the alpha andpolyproline helices and their complexes, the alpha-helicalcoiled-coil, and the pyroline coiled-coils
* Complicated macromolecular formations, including small globularproteins, multidomain proteins and their complexes, and nucleicacids
* Numerous examples of measuring the ground state of proteinenergetics, as well as changes seen when proteins interact
The book also reveals how intertwined structure andthermodynamics are in terms of a macromolecule's organization, mechanism of formation, the stabilization of its three-dimensionalstructure, and ultimately, its function. The first book to describemicrocalorimetric technique in detail, enough for graduate studentsand research scientists to successfully plumb the structuralmysteries of proteins and the double helix, Microcalorimetry ofMacromolecules is an essential introduction to using amicrocalorimeter in biological studies.
Many scientists working in biology are unfamiliar with thebasics of thermodynamics and its role in determining molecularstructures. Yet measuring the heat of structural change a moleculeundergoes under various conditions yields information on theenergies involved and, thus, on the physical bases of theconsidered structures. Microcalorimetry of Macromoleculesoffers protein scientists unique access to this importantinformation.
Divided into thirteen chapters, the book introduces readers tothe basics of thermodynamics as it applies to calorimetry, theevolution of the calorimetric technique, as well as howcalorimetric techniques are used in the thermodynamic studies ofmacromolecules, detailing instruments for measuring the heateffects of various processes. Also provided is general informationon the structure of biological macromolecules, proteins, andnucleic acids, focusing on the key thermodynamic problems relatingto their structure. The book covers:
* The use of supersensitive calorimetric instruments, includingmicro and nano-calorimeters for measuring the heat of isothermalreactions (Isothermal Titration Nano-Calorimeter), the heatcapacities over a broad temperature range (ScanningNano-Calorimeter), and pressure effects (Pressure PerturbationNano-Calorimeter)
* Two of the simplest but key structural elements: the alpha andpolyproline helices and their complexes, the alpha-helicalcoiled-coil, and the pyroline coiled-coils
* Complicated macromolecular formations, including small globularproteins, multidomain proteins and their complexes, and nucleicacids
* Numerous examples of measuring the ground state of proteinenergetics, as well as changes seen when proteins interact
The book also reveals how intertwined structure andthermodynamics are in terms of a macromolecule's organization, mechanism of formation, the stabilization of its three-dimensionalstructure, and ultimately, its function. The first book to describemicrocalorimetric technique in detail, enough for graduate studentsand research scientists to successfully plumb the structuralmysteries of proteins and the double helix, Microcalorimetry ofMacromolecules is an essential introduction to using amicrocalorimeter in biological studies.
