New Frontiers in the Study of Gene Functions

Inhaltsverzeichnis

• 1 The Role of Cis- and Trans-Acting Functions in Simian Virus 40 Gene Regulation.
• 1. Introduction.
• 2. Early Viral Transcriptional Program.
• 3. Late Viral Transcriptional Program.
• 4. Discussion.
• References.
• 2 Regulation of the Heat-Shock Response in Escherichia coli.
• 2. Transcription of the Heat-Shock Genes.
• 3. Regulation of rpoH and ?32.
• 4. Function of the Heat-Shock Proteins.
• 5. Summary.
• 3 Negative Control of DNA Replication Revealed in Composite Simian Virus 40-Bovine Papillomavirus Plasmids.
• 1. Overview.
• 2. Introduction.
• 3. Results.
• 4 DNA Supercoiling as a Regulator of Bacterial Gene Expression.
• 2. Results and Discussion.
• 5 Retrotransposition in Yeast.
• 2. Experiments.
• 3. Speculation.
• 6 Comparative Genetic Analysis of Homeobox Genes in Mouse and Man.
• 2. Murine Homeobox Genes.
• 3. Human Homeobox Genes.
• 4. Comparative Genetic Relationships between Species.
• 5. Comparative Genetic Relationships within Species.
• 6. Possible Functional Relationships between Homeobox Loci and Linked Genes.
• 7. Discussion.
• 7 Eukaryotic Transcriptional Specificity Conferred by DNA-Binding Proteins.
• 3. Concluding Remarks.
• 8 Chromatin Structure Near an Expressed Gene.
• 2. Results.
• 3. Discussion.
• 9 Specificity of Gene Expression and Insertional Mutagenesis in Transgenic Mice.
• 2. Experiments and Results.
• 10 Retroviruses as Insertional Mutagens.
• 2. Insertion of Moloney Murine Leukemia Viruses into the Germ Line of Mice.
• 3. Expression of the Proviral Genome in Mov Substrains ofMice.
• 4. Induction of Two Insertional Mutations.
• 5. Insertional Mutagenesis by Retroviruses and DNA Injection.
• 11 P Transposable Elements and Their Use as Vectors for Gene Transfer in Drosophila.
• 12 Mapping and Manipulating Immunoglobulin Functions.
• 2. Immunoglobulin Gene and Protein Structure.
• 3. Production of Monoclonal Immunoglobulins from Mouse and Man.
• 4. Uses of Specific Immunoglobulins.
• 5. Optimizing Immunoglobulin Structure for Therapy.
• 6. Identifying the Molecular Requirements for Immunoglobulin Synthesis and Function.
• 7. Future Studies on the Structural and Regulatory Mutants.
• 13 T4: A T-Cell Surface Protein Mediating Cell-Cell and Cell-AIDS Virus Interactions.
• 14 Identifying the Determinants of Protein Function and Stability.
• 2. Repressor and Cro Background.
• 3. Isolation of Phenotypically Defective Mutants.
• 4. Structural Distribution of Mutant Sites.
• 5. Why Are Mutant Proteins Nonfunctional?.
• 6. Phenotypic Reversion.
• 7. Summary.