A Laboratory Guide to Biotin-Labeling in Biomolecule Analysis

Inhaltsverzeichnis

• 1 An Introduction to Avidin-Biotin Technology and Options for Biotinylation.
• 1.1 Introduction.
• 1.2 Technical Procedures.
• 1.3 Troubleshooting.
• Acknowledgments.
• References.
• 2 Synthesis of Photocleavable Biotinylated Ligands and Application for Affinity Chromatography.
• Summary.
• 2.1 Introduction.
• 2.2 Technical Procedures.
• 2.3 Results and Discussion.
• 2.4 Troubleshooting.
• 3 Purification of the Receptor for Pituitary Adenylate Cyclase-Activating Polypeptide (PACAP) using Biotinylated Ligands.
• 3.1 Introduction.
• 3.2 Technical Procedures.
• 3.3 Results and Discussion.
• 3.4 Troubleshooting.
• 4 Photoreactive Biotinylated Peptide Ligands for Affinity Labeling.
• 4.1 Introduction.
• 4.2 Technical Procedures.
• 4.3 Results and Discussion.
• 4.4 Troubleshooting.
• 5 Immunoprecipitation of Biotinylated Cell Surface Proteins.
• 5.1 Introduction.
• 5.2 Technical Procedures.
• 5.3 Results and Discussion.
• 5.4 Troubleshooting.
• 6 Biotinylation and Chemical Cross-Linking of Membrane Associated Molecules.
• 6.1 Introduction.
• 6.2 Technical Procedures.
• 6.3 Results and Discussion.
• 6.4 Troubleshooting.
• 7 Preparation of Biotinylated Lectins and Application in Microtiter Plate Assays and Western Blotting.
• 7.1 Introduction.
• 7.2 Technical Procedures.
• 7.3 Results and Discussion.
• 7.4 Troubleshooting.
• Acknowledgements.
• 8 Biotin-Labeling of Poly(ADP-ribose) in Poly(ADP-ribose)-Protein Interactions.
• 8.1 Introduction.
• 8.2 Technical Procedures.
• 8.3 Results and Discussion.
• 8.4 Troubleshooting.
• 9 Preparation and Use of Biotinylated Probes for the Detection and Characterisation of Serine Proteinase and Serine Proteinas Inhibitory Proteins.
• 9.1 Introduction.
• 9.2 Technical Procedures.
• 9.3 Results and Discussion.
• 9.4 Troubleshooting.
• 10 Avidin/Biotin-Mediated Conjugation of Antibodies to Erythrocytes: An Approach for Immunoerythrocyte Exploration in vivo.
• 10.1 Introduction.
• 10.2 Technical Procedures.
• 10.3 Results and Discussion.
• 10.4 Troubleshooting.
• 11 Biotin in vitro Translation: A Nonradioactive Method for the Synthesis of Biotin-Labeled Proteins in a Cell-Free System.
• 11.1 Introduction.
• 11.2 Technical Procedures.
• 11.3 Results and Discussion.
• 11.4 Troubleshooting.
• 12 Nonradioactive Detection of Nucleic Acids with Biotinylated Probes.
• 12.1 Introduction.
• 12.2 Technical procedures.
• 12.3 Results and discussion.
• 12.4 Troubleshooting.
• 13 Biotin-Labeled Riboprobes to Study RNA-Binding Proteins.
• 13.1 Introduction.
• 13.2 Technical Procedures.
• 13.3 Results and Discussion.
• 13.4 Troubleshooting.
• 14 Rapid YAC End Sequencing by Alu-Vector PCR and Biotinylated Primers.
• 14.1 Introduction.
• 14.2 Technical Procedures.
• 14.3 Results and Discussion.
• 14.4 Troubleshooting.