Nonradioactive Labeling and Detection of Biomolecules

herausgegeben von Christoph Kessler

1 General Aspects of Nonradioactive Labeling and Detection.
1.1 Introduction.
1.2 The Concept of Nonradioactive Bioanalytics..
1.3 Direct and Indirect Labeling and Detection Systems.
1.4 Labeling Methods.
1.5 Detection Methods.
1.6 Guide of the Use of Information in this Book.
I Standard Nonradioactive Labeling and Detection Systems.
2 Overview of Nonradioactive Labeling Systems.
3 The Digoxigenin: Anti-Digoxigenin (DIG) System.
4 The Biotin System.
5 In Vivo Labeling of DNA Probes with 5-BrdU.
6 The Sulfone System.
7 Colloidal Gold as a Marker in Molecular Biology: The Use of Ultra-Small Gold Probes.
8 Direct Peroxidase Labeling of Hybridization Probes and Chemiluminescence Detection.
9 A Highly Sensitive Method for Detecting Peroxidase in In Situ Hybridization or Immunohistochemical Assays.
10 The SNAP System.
II Specialized Nonradioactive Detection Systems.
11 Overview of Colorimetric, Chemiluminometric, and Fluorimetric Detection Systems.
12 Colorimetric Systems.
13 Luminescence Systems.
14 Fluorescence Systems.
III Enhanced Systems.
15 Overview of Amplification Systems.
16 Enhanced Signal Generation by Target Amplification.
17 Signal Amplification System: Substrate Cascade.
IV Application Formats.
18 Overview of Application Formats.
19 Blot Formats: Nucleic Acids.
20 Blot Formats: Proteins and Glycoproteins.
21 In Situ Formats.
22 Quantitative Formats.
Appendix I Suppliers of Reagents.
Appendix II Suppliers of Equipment.