Flow Cytometry and Cell Sorting

herausgegeben von Andreas Radbruch

I Flow Cytometry.
1 Operation of a Flow Cytometer.
II Immunofluorescence.
2 Conjugation of Fluorochromes, Haptens, and Phycobiliproteins to Antibodies.
3 Immunofluorescence: Basic Considerations.
4 Multicolor Immunofluorescence Analysis.
5 Combined Intracellular and Surface Staining.
6 Scatchard Analysis by Flow Cytometry.
III DNA and Proliferation.
7 Preparation and Staining of Cells for High-Resolution DNA Analysis.
8 Simultaneous Flow Cytometric Detection of Bromodeoxyuridine Incorporation and Cell Surface Marker Expression.
9 High-Resolution Cell Cycle Analysis: The Flow Cytometric Bromodeoxyuridine-Hoechst Quenching Technique.
IV Cellular Activation and Biochemistry.
10 Cell Activation: Indo-1 Ratiometric Analysis of Intracellular Ionized Calcium.
11 Biochemical Parameters of Cell function.
12 Molecular Analysis of Transcriptional Control: The FACS- Galactosidase Assay.
13 Ligand Acidification by Nonadherent Cells.
V Cell Sorting.
14 Powerful Preselection.
15 High Gradient Magnetic Cell Sorting.
16 Setup of a Flow Sorter.
17 Sorting of Rare Cells.
18 Fluorescence-Activated Chromosome Sorting.
19 Flow Cytometry and Sorting of Plant Chromosomes.
20 Large Particle Sorting.
VI Safety.
21 Biological and Laser Safety.
List of Suppliers.